LOCUS 16c_insert_seque 9484 bp DNA linear UNA 11-JAN-2017 DEFINITION Sequence inserted to create the 16c line of N.benthamiana. ACCESSION urn.local...e-72p0dfl (modified) VERSION urn.local...e-72p0dfl KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature <1..1531 /note="Geneious type: Inserted sequence" /note="Geneious type: misc_feature" source <1532..>6125 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" /note="source synthetic DNA construct" misc_feature 1532..1558 promoter 1621..1800 /note="NOS promoter" /note="NOS promoter;nopaline synthase promoter" CDS 1857..2651 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="aph(3')-II (or nptII)" /note="NeoR/KanR;confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" terminator 3041..3293 /note="NOS terminator" /note="NOS terminator;nopaline synthase terminator and poly(A) signal" promoter 3878..3908 /note="lac promoter" /note="lac promoter;promoter for the E. coli lac operon" protein_bind 3916..3932 /bound_moiety="lac repressor encoded by lacI" /note="lac repressor encoded by lacI lac operator prot" /note="lac operator;The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3921..3943 /note="M13/pUC reverse" /pcr_conditions="60 degree C annealing temperature" /note="M13/pUC reverse" primer_bind 3940..3956 /note="M13 rev primer bind" /note="M13 rev;common sequencing primer, one of multiple similar variants" promoter 4480..4825 /note="35S prom" /note="CaMV 35S promoter;strong constitutive promoter fromcauliflower mosaic virus" misc_feature 4833..4835 source <4846..>4859 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" /note="source synthetic DNA construct" source order(<4846..>4859,<4846..>4859) /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" /note="source synthetic DNA construct" misc_feature 4846..4855 /Note="a ribosome binding site (RBS) for bacterial expression" misc_feature 4856..4859 CDS 4860..4925 gene 4860..5651 /note="modified green fluorescent protein mGFP5-ER containing one nucleotide variation resulting in an H46Y amino acid change" misc_feature 4995..4997 CDS 5637..5648 terminator 5668..5920 /note="NOS terminator" /note="NOS terminator;nopaline synthase terminator and poly(A) signal" misc_feature 5808..5810 misc_feature complement(5933..5949) /note="Geneious type: primer_bind_reverse" /note="M13 fwd primer bind" /note="M13 fwd;common sequencing primer, one of multiple similar variants" misc_feature complement(5942..5964) /note="Geneious type: primer_bind_reverse" /note="M13/pUC forward" /note="M13/pUC forward" misc_feature 6118..6125 mobile_element 6126..>9366 /mobile_element_type="transposon:Tn5393" repeat_region 6126..6206 /note="5' inverted terminal repeat; putative" /rpt_type="inverted" source complement(<6126..>9366) /organism="Agrobacterium tumefaciens LBA4213 (Ach5)" /mol_type="genomic DNA" /strain="LBA4213 (Ach5)" /db_xref="taxon:1435057" /plasmid="pTi" /note="tumor-inducing plasmid" source 6126..>9366 /organism="Erwinia amylovora" /mol_type="genomic DNA" /db_xref="taxon:552" /plasmid="pEa34" gene complement(6159..9044) /gene="tnpA" gene 9239..>9366 /gene="tnpR" misc_feature 9364 misc_feature 9367..9484 /note="Geneious type: Inserted sequence" /note="Geneious type: misc_feature" misc_feature 9407 ORIGIN 1 gccctccatt attcactacc tcgaaacatt gacagaggat ttgttcactg cacgatttgc 61 agactgtcga ttcgatgaga caatttttcc caaaattagg ggagaagttg gtggtgaaac 121 caaacgggaa attttgtgaa aaaatcaatc attatctcat cttgatccgt gtgcctctat 181 ttgttaaaaa taggtgcaaa agattattca tttgcaaaga atagcaaatc aaatgccaca 241 cacatttacg gatttgaaaa ggataacaaa attatatatg cccgcagaga atgtgatagc 301 agtatgtgat aagtctgaaa gaagataaaa ttattaccgt gaaggtatca atggacgtgg 361 acgtgacaat agacgaaatt ataatcgtca tcattgtgat aatgagaata ataagaattc 421 tcaaaataat ccttcacttt gtaaaagtaa tatctgtcat cgatttgaca tatattttat 481 atatcacata tatatgatta tggtccattc atggtgtgaa tgtgacaaca tctaatttat 541 gaatacatat tcattattgg aagaatatga acatgctatt gtattgatta taccacactc 601 acctctagaa tgagagttga gatgatataa gaaaacaatg tcattattat gacatgaatg 661 gtcattggtc acgtacctat tgtacgccaa aatattttgg caatgtgatt attaaaggac 721 aataataatg caagaaacat atcttgcata taattttgac atgaccataa tggtacaact 781 ttctctttaa aagaaatatt cactatattg atattgatta atatgtgata gcaccaaatt 841 aattgagtac tctgtaagag ccaattatat tattttggag aaacacaatt gattaccaat 901 aaggtattgt attgtagtaa gtctcaaaga aacttattca gtttccaaag tatacgcgaa 961 agcgattgat tatattgaga ctgtaaataa aggaaagatt tgatatctgc ttttactaca 1021 acttgtagcg ggataatatg tatgtgaaag ctacccactt tattctccaa tttgtactat 1081 acaaacaaaa gaattccaca ataaagtgga tgattattga tataaaaact aatatcataa 1141 taaatttaga gtttatcaaa aattgcacat gtcatgatgt aaacctgaat tttatcaata 1201 ttgataattt attcagttgg cattcagatt agccatatca atttaagtat ggtatgcaac 1261 aataatagag aattcatatg ggtaaatatt aaagaactag aaaattcttt acgaattctc 1321 ttgtatttct tgttctcatt aattaattga ccaactaaag ttggggttga gtcccaaaaa 1381 ttctagaaat atcaaaggtg aatatgggcc tattcacctg ccatgtatac cacataagat 1441 gcatcaatga gatggttaca tgtgcgatta ttattaacct gcaacctggc cggtgcttgc 1501 gaggtaactt gctcagtaat ctgatttcga gcaatctgat catgagcgga gaattaaggg 1561 agtcacgtta tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca 1621 gaaccgcaac gttgaaggag ccactcagcc gcgggtttct ggagtttaat gagctaagca 1681 catacgtcag aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca 1741 aatatttctt gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta 1801 gagtctcata ttcactctca atccaaataa tctgcaccgg atctggatcg tttcgcatga 1861 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 1921 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 1981 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 2041 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 2101 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 2161 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 2221 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 2281 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 2341 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 2401 atgatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 2461 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 2521 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 2581 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 2641 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 2701 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 2761 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 2821 cgggatctct gcggaacagg cggtcgaagg tgccgatatc attacgacag caacggccga 2881 caagcacaac gccacgatcc tgagcgacaa tatgatcggg cccggcgtcc acatcaacgg 2941 cgtcggcggc gactgcccag gcaagaccga gatgcaccgc gatatcttgc tgcgttcgga 3001 tattttcgtg gagttcccgc cacagacccg gatgatcccc gatcgttcaa acatttggca 3061 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct 3121 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg 3181 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata 3241 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggcctc 3301 ctgtcaatgc tggcggcggc tctggtggtg gttctggtgg cggctctgag ggtggtggct 3361 ctgagggtgg cggttctgag ggtggcggct ctgagggagg cggttccggt ggtggctctg 3421 gttccggtga ttttgattat gaaaagatgg caaacgctaa taagggggct atgaccgaaa 3481 atgccgatga aaacgcgcta cagtctgacg ctaaaggcaa acttgattct gtcgctactg 3541 attacggtgc tgctatcgat ggtttcattg gtgacgtttc cggccttgct aatggtaatg 3601 gtgctactgg tgattttgct ggctctaatt cccaaatggc tcaagtcggt gacggtgata 3661 attcaccttt aatgaataat ttccgtcaat atttaccttc cctccctcaa tcggttgaat 3721 gtcgcccttt tgtctttggc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 3781 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 3841 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 3901 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 3961 attacgccaa gcttgcatgc ctgcaggtcc ccagattagc cttttcaatt tcagaaagaa 4021 tgctaaccca cagatggtta gagaggctta cgcagcaggt ctcatcaaga cgatctaccc 4081 gagcaataat ctccaggaaa tcaaatacct tcccaagaag gttaaagatg cagtcaaaag 4141 attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac 4201 tattccagta tggacgattc aaggcttgct tcacaaacca aggcaagtaa tagagattgg 4261 agtctctaaa aaggtagttc ccactgaatc aaaggccatg gagtcaaaga ttcaaataga 4321 ggacctaaca gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact 4381 caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa 4441 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt 4501 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat 4561 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt 4621 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt 4681 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac 4741 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg 4801 aagttcattt catttggaga gaacacgggg gactctagag gatccaagga gatataacaa 4861 tgaagactaa tctttttctc tttctcatct tttcacttct cctatcatta tcctcggccg 4921 aattcagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg 4981 gtgatgttaa tgggtacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg 5041 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac 5101 ttgtcactac tttctcttat ggtgttcaat gcttttcaag atacccagat catatgaagc 5161 ggcacgactt cttcaagagc gccatgcctg agggatacgt gcaggagagg accatcttct 5221 tcaaggacga cgggaactac aagacacgtg ctgaagtcaa gtttgaggga gacaccctcg 5281 tcaacaggat cgagcttaag ggaatcgatt tcaaggagga cggaaacatc ctcggccaca 5341 agttggaata caactacaac tcccacaacg tatacatcat ggccgacaag caaaagaacg 5401 gcatcaaagc caacttcaag acccgccaca acatcgaaga cggcggcgtg caactcgctg 5461 atcattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt 5521 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc 5581 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaacatg 5641 atgagcttta agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta 5701 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt 5761 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt 5821 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag 5881 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattaat tcactggccg 5941 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 6001 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 6061 aacagttgcg cagcctgaat ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc 6121 acgttggggt cgtttgcggg agggggcgga atcctacgct aaggctttgg ccagcgatat 6181 tctccggtga gattgatgtg ttcccatccg agcggcgaaa catgggccaa gagatcgggc 6241 gatagcagct ttccatcgcg tttctggttt gcaacgacct cgccgagctt catggtgttc 6301 cagaagatga tgatggcggc gagcagattc atgccggcga tgcggtaatg ctggccttcg 6361 gcggaacggt cgcggatttc accgcggcgg tggaagctga ttgcccgctt cagcgcatga 6421 tgagcttcgc ctttgttgag cccgatctgg gcacgccgtt ggagttcggc atccagaatc 6481 cagtcgatca tgaacagggt gcgctcgacg cgaccgactt cccgcagggc tgtcgcgagc 6541 tcgttctgcc gcggatagga ggcgagtttc cgcagaatct ggcttggcgc gacggtcccg 6601 gcagcaatgg tggcggcgat gcgcaggatg tcgggccaat tgcgctcgat catggcttgg 6661 ttgacctttc cgccgatcaa cgctcgcagg tgcgccgggg cggccgacgg attgaacgcg 6721 tagagccgtt tggatggcag gtcgcggatg cgcggagcga accggtagcc gagaatggca 6781 catgcggcaa agacgtgatc ggtgaagccg cccgtgtcgg tgaactgctc gcggatatgg 6841 cgtccagcat cgttcatcag caggccatcg aggatgtaag gcgcttcgct tgccgttgca 6901 ggaatcacct gggttgcgaa cggcgcatat tggtcggaga cgtggctata ggctttcagg 6961 cccggggtat tgccatattt cgcgttgacc aggttcatgg cctcaccttg ctctgtagcg 7021 acgaagaact gtccgtcgct cgaagccgac gtgcccatgc cccagaaccg ggccatgggt 7081 aacgctgcct gtgcctcgac caccatggcc agcgcccggt cataggcttc gccctcgaca 7141 tgccaccgtc caatgcggat caattcccag aaggtgtggg tgtttgtcgc atccgccatt 7201 ttgcgcaagc cgaggttgat cccttccgcc aagataacgt tcattagccc gatccggtca 7261 gcgcagggtg ctcctgtgcg cagatgggtg aacgcttcgg tgaagccggt cgccgcatcc 7321 acctccagca ggagatcggt gatgcgcgtg ggcgggatct gcttgtagag atcgagcacc 7381 agatcttcgg cgcctgtcgg cgcggcggct tcgagtttct cgatatgcag aacgccgttt 7441 tcaatcgacc cgcccgggat cgtgcctgcg cgagcggcac ggccaagctc gcgcaaccgc 7501 atgtcgaggc gagcttgccg gtctgccagc cattcctccg gccgcaatgg cacagcgaga 7561 cgaccgcctt ccgcgatgga ttgtgccgga acgagtgcgt gtttcagatc gccatagcgc 7621 cgggacctag taagccagac atctccggag cggaacgcat cgcgcagatg gaacagcacc 7681 gcgatctccc ataggcgagc gtcgccagcc ctctgggccc gaaggtggcg atgccatttc 7741 gagctgggcc gcaagaagct ggtcatcgcg gcatcgttca aaccggtacg aagggccgtc 7801 accgcttcca gaagcggcag tgcaacgggc gcagctcgca gatcgagcag gcgcaacatg 7861 cgtggagcgt atcggcggaa gcggtgataa ccgtcgagca catgattgag cggatcgtcg 7921 gccatggtgg cggtcagcct ggttgccatt gcaacaaggg tttttaagcc gtcccaccct 7981 gacccactcg cgatgacatc gcccagcggc tggccatcat cctgtgcatc gaccagggcg 8041 cccccgatct cggcgaagga tttcagggtg tcacgcacca cccccgcttc gtctgcgacc 8101 tttgcatggc aaatacgctc cgaagcacgg tagagacggc cgacgatccg gtcgtgggtt 8161 tcgaccactg cgtcggccaa catcgcctgc cattccgaga cgcaaacagc caagatcgca 8221 agccgcctgt cctccgggag atcgcgcatg ccgtcggcat aataccgttc accctgcctg 8281 cgcagacgag tcacccgatg ggcaggaacg ccggcaagca gatcctcggg gagatcgatg 8341 cgttgcagat attcgagccg gtcgagcagc cggttggccg acgaagagtt cgagccaggc 8401 tcgaactggc gcagccacac aaaacgggtc acccgatcat cagccgtctc ctcgagcaat 8461 gccagcaact gttctcggat cgacataggc agccgactgg cgatcctcgt ctcgatgcgt 8521 cgctcggcat cgacgagagc cgcggcacaa agccgctcga tcgtggatgt cgcgggaagg 8581 acagtgcggg tgcgtcggca ctcggctacg aagcgacggg cgatatcctc gttcgacacc 8641 gccatctcgg cttctcggaa caaccattcc ttcagctcgc tcgcaccacg tccggagaag 8701 gtgcggaagc cgtagagccc ccgtaactcg gcaagatgct cgtgccgtgt ttcctcgcgg 8761 gcagcatagt ctacgagatc gtcggcaccc aggccaagct gcgctccgat aaattcgatg 8821 acctctgcag ggatcagttc gcctggagcc agcacccggc cgggatagcg caggacacac 8881 aattgcaggg cgaagccgaa cctgttgtga gcgcgccgac gcagcctgat atgcccaagg 8941 tcttcatcac tcagcgtata gtgcttgagc aaatccgtct gtgaagtcgg caagcgcaac 9001 agcgcgtctt tctgccgatc ggttagagtg acgcgacgcg gcatacatgt tcctttttca 9061 aaatctgata gcgttcaaga cgctttgttt atgaagctgg ttgagataca tttccagagg 9121 tcaatgcaat cgtggccgaa gcgccgcctc aaaccaacgt ttgtgataca tgctgatcgg 9181 atatgcccgc gtctccaaag ccgatggctc gcagtctctc gacctgcagc acgacgcctt 9241 gcgcgccgca ggtgtcgaac gggacaatat ctatgatgat cttgcttccg gcggtcgtga 9301 tgatcgccct ggcttgactg cctgcctcaa gtcattgcgt gacggcgatg tgctggtggt 9361 ctgaaattgg tttagcaaaa taatttattg agtgcctcca ctaaatagct aaactattgc 9421 ttatgagaac aaagttatct atatcaattt gatatacgtt atatacgaga gtatattata 9481 ttct //