The T-DNA locus of N.benthamiana 16c
mGFP5-ER sequence
Tn5393 sequence
Whole insert sequence in 16c
GFP-ER transcript in 16c
Insertion point on LAB genome
Sequence Deleted from Genome at T-DNA insertion site in Scaffold 4095
Download annotated whole insertion sequence (Geneious Format) Download annotated whole insertion sequence (Genbank Format)
The details of how the insertion sequence was obtained and verified are described in Philips et al 2017
Primer ID Sequence Location
Primer A
AGGAATATATGTTGGGTTTGAATC
N. benthamiana flanking RB in 16c F2
Primer B
AATTCTGGAAATATCAAAGGTG
N. benthamiana flanking RB in 16c F1
Primer C
GCTTAGCTCATTAAACTCCAGA
NOS promoter R
Primer D
CTCGGCCACAAGTTGGAATA
Internal primer for mGFP5-ER F
Primer E
GGTCTTGAAGTTGGCTTTGATG
Internal primer for mGFP5-ER R
Primer F
CACTTGTGAGGGGAGAATATAA
N. benthamiana flanking LB in 16c R
| Primer ID | Sequence | Location |
|---|---|---|
| Primer A | AGGAATATATGTTGGGTTTGAATC | N. benthamiana flanking RB in 16c F2 |
| Primer B | AATTCTGGAAATATCAAAGGTG | N. benthamiana flanking RB in 16c F1 |
| Primer C | GCTTAGCTCATTAAACTCCAGA | NOS promoter R |
| Primer D | CTCGGCCACAAGTTGGAATA | Internal primer for mGFP5-ER F |
| Primer E | GGTCTTGAAGTTGGCTTTGATG | Internal primer for mGFP5-ER R |
| Primer F | CACTTGTGAGGGGAGAATATAA | N. benthamiana flanking LB in 16c R |
